T7

Part:BBa_K1132045:Experience

Designed by: Isabelle Te   Group: iGEM13_INSA_Toulouse   (2013-09-18)


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Results of BBa_K1132045 characterization

Following is the electrophoresis gel showing that we obtained a fragment of expected length (829 bp) by restriction with EcoRI/PstI: Result_polT7_2.jpg

Verifications of the validity of the constructions were performed with visual inspection of the colours of the colonies. The pT7-RFP plasmid was transformed into competent BL21-DE3. Plates were supplemented or not with IPTG. White colonies were obtained on the Petri dishes with no IPTG and red colonies on the Petri dishes containing IPTG.

Boite_rouge.jpg

Boite_blanche.jpg

The biobrick behave as expected.

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